Anomalous nuclease digestion of Holothuria, sperm chromatin containing histone H1 variants
نویسندگان
چکیده
منابع مشابه
Distribution of H1 histone in chromatin digested by micrococcal nuclease.
The relative amount of H1 histone associated with isolated nucleosomes from calf thymus was determined as a function of the extent of DNA digestion by micrococcal nuclease. Generally the amount of H1 histone associated with mononucleosomes decreases with increasing digestion until 60% of the original H1 remains associated with DNA 150 base pirs or less in size. Coincidentally, H1 histone increa...
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Condensed and late-replicating interphase chromatin in the Dipertan insect Chironomus contains a divergent type of histone H1 with an inserted KAP-KAP repeat that is conserved in single H1 variants of Caenorhabditis elegans and Volvox carteri. H1 peptides comprising the insertion interact specifically with DNA. The Chironomid Glyptotendipes exhibits a corresponding correlation between the prese...
متن کاملHistone H1 affects the loss of chromatin fragments from nuclease-treated nuclei.
During nuclease treatment of nuclei in 80 mM buffers, a frequently used technique for solubilizing chromatin, oligonucleosomes escape the nucleus and are commonly discarded. These released oligonucleosomes lack H1 histone. Their loss of histone is easily explained by the recently described exchange of H1 that takes place at such ionic strengths. A comparison of H1-depleted nuclei to complete nu...
متن کاملHistone H1 gets Pin’d onto chromatin
Histone H1 gets Pin'd onto chromatin T he proline isomerase Pin1 limits chromosome decon-densation by stabilizing a histone's association with chromatin, Raghuram et al. reveal. Histone H1 incorporates into nucleosomes to enhance chromatin folding and condensation. Phosphory-lation by the cyclin-dependent kinase Cdk2 promotes H1's dissociation from chromatin, perhaps by altering the conformatio...
متن کاملXenopus Egg Extracts Increase Dynamics of Histone H1 on Sperm Chromatin
BACKGROUND Linker histone H1 has been studied in vivo and using reconstituted chromatin, but there have been few systematic studies of the effects of the cellular environment on its function. Due to the presence of many other chromatin factors and specific chaperones such as RanBP7/importin beta that regulate histone H1, linker histones likely function differently in vivo than in purified syste...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1985
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1985.tb08871.x